Coding

Part:BBa_K737003:Design

Designed by: Lei Chang   Group: iGEM12_OUC-China   (2012-09-18)


This part is polyphosphate kinase.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1665
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This coding contain a EcoRⅠenzyme cutting site should be removed, so it could only be cut in XbalI and PstI sites.


Source

We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we design by ourselves.

References